Assessing binding of WT ectopically expressed 3xFLAG-UBE2H and its C-terminal extension mutants to endogenous CTLH. Immunoblots detect the core (RANBP9) and catalytic (MAEA) CTLH subunits as well as various 3xFLAG-UBE2H versions in the input HEK293 lysate and samples after FLAG IP. See alsoFigure S4.
