Gel from Scientific Research
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In vitro assays probing the roles of phosphorylated Ubc8/UBE2H C-terminal extension for ubiquitylation of the fluorescent model peptide substrates (pep∗) designed as described inFigure2B and harboring either Mdh2 degron or human GID4 recognition sequence PGLWRS at their N termini for reactions mediated by GIDSR4(left) and CTLHSR4(right), respectively.
#Gel#In Vitro Assays#Ubiquitylation#Ubc8#UBE2H#C-Terminal Extension#Peptide Substrates#GIDSR4#CTLHSR4
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