Line Plot from Scientific Research

Intact mass spectrometry analyses revealing multisite phosphorylation of insect cell (IC)-expressed C-terminally Twin-Strep-tagged yeast Ubc8 (left) and human UBE2H (right) compared with their unphosphorylated lambda phosphatase-treated (PP) and bacterially expressed (BC) versions. 40-Da difference between the BC and PP-treated IC E2s stems from N-terminal acetylation of the latter.

DOI