Segmented 3.5-A resolution focused refined map of Ubc8~ubiquitin-bound Gid2-Gid9, sharpened with DeepEMhancer (left). The corresponding model (right) illustrates conserved and unique E3-E2~ubiquitin interactions mediating recruitment of ubiquitin-conjugated Ubc8s UBC domain (top) and its allosteric activation (bottom). The bowl-shaped cavity cradling the UBC domain is marked with a black dashed line.
