SMT of N-terminal TFID (left) and C-terminal TFID (center) constructs in the presence (lighter hue) or absence (darker hue) of A485, with bar plot offbound(right). Note that in both (C) and (D), the FL data are those identified as WT Transgene inFigure 1and WT in (A) and (B). SMT: bars represent bootstrapping mean SD while p values are reported as ns, (not significant),*p < 0.05,**p < 0.01, or***p < 0.001. FRAP: bars represent best fit 95% CI. See alsoFigure S5.
