Levels of iRhom2 and CtBP proteins by immunoblotting (J) and transcript levels of indicated target genes by qPCR (K) were analyzed in HEK293T cells expressing inducible human HA-iRhom2 for 4 h and transfected with control siRNA and combined CtBP1/CtBP2 siRNAs. Data are presented as log2-fold change relative to uninduced cells as mean ± SEM, n = 3.
