Islets from AdarKO mice were isolated 3 days after tamoxifen injection and cultured for 3 additional days in RPMI medium supplemented with 5 or 11 mM glucose and pharmacological modulators of insulin secretion, as indicated: 10 μM nifedipine. Each dot represents RNA expression levels from 30 islets isolated from AdarKO mice (n 3). ISG mRNA expression was assayed by RT-qPCR and normalized to Actb gene expression.
