Representative images ( g ) and signal quantification ( h ) of proximity ligation assay (PLA) detecting methylated BCAR3 by coupling antibodies against HA-tagged total BCAR3 and BCAR3 K334me1 in MDA-MB-231 cells carrying indicated modifications. Dotted red lines represent cells periphery and dotted white square represents the enlarged area depicted in Supplementary Fig. S2k . P -values were calculated by ANOVA with Tukey’s testing for multiple comparisons. Scale bars, 10 μm.
