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Open access visualization of Microscopy, Cell Structures, BMP4 Inhibition, WNT Inhibition, Extra-embryonic Lineages
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Representative images showing structures formed when BMP4 (LDN193189 200 nM) or WNT (XAV939 5 M) signaling was inhibited in the first 48 h of extra-embryonic lineages induction. Left: z-stack section of light-sheet imaging at day 8. Scale bars, 100 m. Right: bright-field (BF) images and corresponding percentage of structures that form amniotic sac-like structure at day 15.n= 64 structures were analyzed per experimental condition. Scale bars, 400 m.

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