Evaluation of INSRTR regulation on representative proteins from various protein groups, each tested for a corresponding specific protein functional assay. ( k ) Fluorescent protein GFP. Inserts are schematic presentations of allosterically regulated protein 3D structures with highlighted each peptide insertion site (red sphere). Mock represents transfection with reporter plasmids only. The values in ( a – k ) are the means of four biological replicates ± SD and representative of three independent experiments on transiently transfected HEK293T cells. Statistical significance was tested with one-way ANOVA with Dunnett’s multiple comparisons test between the INSRTR variant and the addition of a REG peptide; P values 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****) (significance, confidence intervals, degrees of freedom, F and P values are listed in Supplementary Table S2 ).
