Evaluation of INSRTR regulation on representative proteins from various protein groups, each tested for a corresponding specific protein functional assay. Enzymes: ( b , c ) TEV protease. Inserts are schematic presentations of allosterically regulated protein 3D structures with highlighted each peptide insertion site (red sphere). Mock represents transfection with reporter plasmids only. The values in ( a – k ) are the means of four biological replicates ± SD and representative of three independent experiments on transiently transfected HEK293T cells. Statistical significance was tested with one-way ANOVA with Dunnett’s multiple comparisons test between the INSRTR variant and the addition of a REG peptide; P values 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****) (significance, confidence intervals, degrees of freedom, F and P values are listed in Supplementary Table S2 ).
