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Schematic presentation of the inverted INSRTR (ON switch) design. The ON-INSRTR was designed by genetic fusion of a low-affinity CC peptide (inhibitory peptide INH) to the C-terminus of the target protein with an inserted peptide in the protein loop (inserted peptide INS). Due to the high intramolecular concentration, the inhibitory and inserted peptides form an intramolecular CC dimer despite low affinity, resulting in an inactivated target protein. The addition of the REG, with a high affinity for an inhibitory peptide, results in an unstructured inserted peptide, thus regaining target protein function.

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