Different N7 peptide delivery systems were evaluated for regulation of the ON-INSRTR system. The activity of ON-INSRTR firefly luciferase is upregulated with the co-expression of REG peptide N7. The values are the means of four biological replicates SD and representative of at least two independent experiments on transiently transfected HEK293T cells. Significance was tested with one-way ANOVA with Dunnett’s multiple comparisons test between the INSRTR variant and the addition of a REG peptide; P values 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****) (significance, confidence intervals, degrees of freedom, F and P values are listed in Supplementary Table S2 ).
