Coupling of chemical regulation to the INSRTR, through chemically activated protease regulation of ON-INSRTR switch, mediated by cleavage of the linker peptide between INH and the target protein. The values are the means of four biological replicates SD and representative of at least two independent experiments on transiently transfected HEK293T cells. Significance was tested with one-way ANOVA with Dunnett’s multiple comparisons test between the INSRTR variant and the addition of a REG peptide; P values 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****) (significance, confidence intervals, degrees of freedom, F and P values are listed in Supplementary Table S2 ).
