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Open access visualization of Illustration, Workflow, Protein Structure, Microscopy, Cyclic Oligomer
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Starting from the design model of a C 3 cyclic oligomer with the bonding motif, we generated five distinct complementary assembly partners that generate different closed architectures. From left to right: D32-12 (dimeric partner no. 1), O32-17 (dimeric partner no. 2), I32-2 (dimeric partner no. 3), O43-14 (tetrameric partner no. 4) and O43-36 (tetrameric partner no. 5). nsEM (bottom) single-particle views, 2D class averages and 3D reconstruction are consistent with the design models. We use the same procedure recursively to generate building blocks complementary to the newly designed components: the O43-9 cage (far right) is derived from the secondary C 4 component of the O43-36 assembly.

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