Bar Plot from Scientific Research

RelA-mediated NF-κB activation in response to rupintrivir, which preserves the function of iCROP-engineered RelA (left). RelA bearing both the HRVp and CS at position S319 is fragmented into two non-functional parts, unless the protease inhibitor is provided, when transcription of the reporter SEAP can occur. HEK293T cells were co-transfected with constitutively expressed iCROP-RelA (P mPGK -N-RelA-HRVp-RelA-C-pA) and P NF-κB -SEAP-pA and treated with rupintrivir at various concentrations for 24 h before analyzing SEAP expression levels (right). Data are shown as mean SD ( n = 3 biological replicates). Statistical significance was calculated by means of Welch’s two-tailed t -test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant.

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