Line Plot from Scientific Research

BLI binding traces (associations and dissociations phases) obtained with streptavidin sensors loaded with biotinylated 2 -microglobulin. The association was monitored in wells containing 25, 50, 100, 200 and 400 nM of Nb24 nanobody variants (legend). Data were fitted globally with a 1:1 partial dissociation binding model (solid lines) using R max, on rate and off rate as global parameters and Y tinf as local parameter.

DOI