Line Plot from Scientific Research

MTT assay results of 3T3 and 3T3 CPD cells exposed to H 2 O 2 concentrations ranging from 200 to 3000 µM. Mean percentage ± SEM ( n = 8 biologically independent samples) cell viability with respect to untreated cells is plotted on the y-axis, while H 2 O 2 concentrations are indicated on the x -axis. Controls confirming that the transfection and subsequent selection procedures are not responsible for the observed results with the 3T3 cells are provided where mutant forms of CPDphr were ectopically expressed in 3T3 cells and results equivalent to those from untransfected 3T3 cells were obtained (see below). All experiments were conducted in constant darkness (DD) and repeated at least 3 times, independently. For the cell viability assays ( b , c ), representative data is shown. The statistical test used for ( a ) is student’s t -test, while the analysis for ( b , c ) is two-way ANOVA analysis. Statistical differences ( P values) are indicated in each panel. Source data are provided as a Source Data file.

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