Workflow from Scientific Research

BioPKS pipeline uses both multifunctional type I polyketide synthases (PKSs) and monofunctional enzymes for chemical biosynthesis. Our platform is built upon two key rule-based components RetroTide and DORAnet. When a user inputs a target structure, RetroTide first suggests chimeric PKS designs to synthesize the carbon backbone of this target. DORAnet then performs enzymatic post-PKS decorations of the PKS product to reach the target chemical. If an integrated PKS and post-PKS pathway design is found, the user can collect this design from BioPKS pipeline for future experimental synthesis in the laboratory.

DOI