Gel from Scientific Research

cpzA3H-7KR contains K27R, K50R, K51R, K52R, K153R, K161R, and K168R mutations and protects cpzA3H from both NL4-3 Vif (left panel) and LAI Vif (right panel) induced degradation (lane 2). Each mutation was reversed within cpzA3H-7KR to generate cpzA3H-7KR-R27K, -R50K, -R51K, -R52K, -R153K, -R161K and -R168K (lanes 3 to 9). The 7KR + R50K (lane 4) and 7KR + R51K (lane 5) variants showed protein degradation efficiency equivalent to wild-type cpzA3H (lane 1). All proteins were expressed without any tag and detected by anti-A3H rabbit polyclonal Abs.

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