Workflow from Scientific Research

Open access visualization of Workflow, Illustration, dCas9-P300 cassette, Shox2 promoter, sgRNAs
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A dCas9-P300 cassette was inserted as a sensor upstream of the Shox2 promoter, two sgRNAs to target dCas9 activity were integrated at the ColA1 safe harbour locus through an FRT-mediated recombination.

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