Microscopy from Scientific Research

Representative images of Swiss-roll colon sections from 8- to 12-week-old mice with the indicated genotypes stained with bacterial 16S rRNA in situ hybridization probe EUB338 (yellow). Nuclei were stained with DAPI (aqua). Each colonic structure and layer is labeled with white dashed lines. C, crypt; L.P, lamina propria; M.m, muscularis mucosae; S.M, submucosa; M.e, muscularis externa. White arrows mark bacteria. Bar graph depicting the percentage of 8- to 12-week-old mice with indicated genotypes that contain bacteria in the submucosa layer (right). Scale bars, 10 μm.

DOI