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Representative segmentation analysis of a dynamic smFRET trace reveals the cycle time ( cycle ) of nNE openingclosing events, subdivided into low-FRET-state time ( O ), transition time from low- to high-FRET ( t-C ), high-FRET-state time ( C ), and transition time from high- to low-FRET ( t-O ). Green arrows, abortive transcription events.
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