Comparison of the angle distribution under the condition of transcription and no transcription. Red, distribution of angles in nanoengines deposited on TEM grids that did not undergo transcription ( n =5,135, 189 micrographs, 64.13° ± 20.04°, min = 0.00°, max = 161.11°, median = 67.03°); cyan, distribution of angles in nanoengines that underwent transcription deposited on TEM grids ( n = 3,266, 99 micrographs, 56.51° ± 21.79°, min = 0.00°, max = 132.64°, median = 58.43). The angles were measured using the angle measurement tool of ImageJ software (Supplementary Dataset 4 , Extended Data Fig. 4 and Supplementary Table 1 ). Percentages of relative counts were calculated by dividing the counts of each bar (bin width, 3°) by the total amount of data in the population.
