Workflow from Scientific Research

Schematic of the zebrafish model, which expresses the enzyme nitroreductase (NTR) under the control of the insulin promoter and converts the MTZ compound into a toxic byproduct that ablates -cells. Larvae were treated with MTZ at 2 dpf for 24 h. Double-transgenic larvae, Tg ( ins : CFP - NTR ); Tg ( ins : Kaede ), were used to visualize -cells with the fluorescent protein Kaede. NR K-PEG , NR-1 K-PEG or NR-7 K-PEG was intravenously injected into larvae at 3 dpf and the measurements of free glucose levels were taken 4 hpi.

DOI