Ultrasound images ( b ) and SBR quantification ( c ) of normal and fibrosis cohorts. The B-mode images reveal the position of the liver, AM images show the GV signal inside the liver, and the MMUS images (parula) were overlapped with Doppler images (grey) to show the signal below the skin. P = 0.00033411. n = 4 for normal group, n = 7 for fibrosis group. Each data point represents biologically independent animals, and lines represent the median of each group. Significance was determined using an unpaired two-sided t -test: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. In b min and max on the parula (MMUS) and hot (AM-mode) colour bars represent 0 and 500,000, respectively, and the grey (B-mode) colour bar ranges from 0 to 1,000,000 arbitrary units.
