Microscopy from Scientific Research

Representative TIRF micrographs of Alexa488-FtsZ at increasing FtsZ and constant FtsA concentrations. Below 0.625 µM FtsZ, filaments do not form higher-order structures. At 1.25 and 1.50 µM, FtsZ forms rotating rings and directionally moving filament bundles (see red squares). This organization is lost at concentrations above 3 µM FtsZ, at which filaments densely cover the membrane surface. The micrographs are representatives of at least four independent experiments ( n = 4, 6, 8, 5 and 4 for [FtsZ] = 0.625, 1.25, 1.5, 3.0 and 5.0 µM, respectively).

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