The total number, distribution and origin of variants detected by serial liquid biopsy analyses are shown for 45 patients with at least two serial liquid biopsies performed. A WBC DNA-informed approach allowed for classification of plasma variants by cellular origin and revealed that 17% of the plasma variants detected (14/82 variants) could be attributed to clonal hematopoiesis mutations. Frequently mutated genes included TP53 , KRAS , ARID1A , ATM , NRAS and PDGFRA , which is consistent with the reported genomic landscape of NSCLC. Alteration prevalence for each gene is listed on the right. The mutation count per sample is displayed at the top, followed by rows indicating sample timepoint and ctDNA molecular response. Ten patients had undetectable tumor-derived mutations at all timepoints, rendering 35 patients evaluable for ctDNA molecular response. NE, not evaluable.
