After completion of shaping tasks (see Methods for details), the visual discrimination performance of 4-month-old WT, zQ175, CR3 KO and zQ175 CR3 KO mice was assessed using the Bussey–Sakisda operant touchscreen platform. Line charts show performance over 11 trial sessions (60 trials per session) of (i) WT and zQ175 mice, (ii) zQ175 and zQ175 CR3 KO mice and (iii) all groups, n = 29 WT mice, 18 zQ175 mice, 24 CR3KO mice and 13 zQ175 CR3KO mice. Two-way ANOVA: for WT versus zQ175 P = 0.038 for the combination of genotype trial session as a significant source of variation and P = 0.01 for genotype as a significant source of variation; for zQ175 versus zQ175 CR3KO P = 0.069 for the combination of genotype trial session as a significant source of variation and P = 0.568 for genotype as a significant source of variation; for WT versus zQ175 CR3KO P = 0.352 for the combination of genotype and trial as a significant source of variation and P = 0.068 for genotype as a significant source of variation; for WT versus CR3 KO P = 0.319 for the combination of genotype trial session as a significant source of variation and P = 0.593 for genotype as a significant source of variation.
