Microscopy from Scientific Research

(I), Representative confocal images of CR3 and Iba1 staining in the dorsal striatum of 3-month-old zQ175 and WT mice. Although levels of microglia (and macrophage) marker Iba1 do not change (Extended Data Fig. 4g ), CR3 levels are increased in the process tips of microglia from zQ175 mice. Scale bar, 10 m. h (II), SIM image showing CR3 localized to the process tips of microglia in the dorsal striatum of zQ175 mice. Scale bar, 10 m. Bar chart shows quantification of average CR3 fluorescence intensity in the dorsal striatum, n = 4 WT and 3 zQ175 mice. Unpaired two-tailed t -test, P = 0.034. For bar charts, bars depict the mean. All error bars represent s.e.m. * P < 0.05, ** P < 0.01 and *** P < 0.001. mo, months old. IF, immunofluorescence.

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