Quantification of the percentage of VGLUT1 + glutamatergic synapses associating with C3 and C1q puncta in the caudate nucleus and cerebellum of postmortem tissue from patients with HD assessed to be either Vonsattel grade 2 or Vonsattel grade 4 relative to that seen in tissue from control individuals. For C3 in the caudate nucleus samples, n = 6 control individuals, n = 4 HD individuals with Vonsattel tissue grade 2 and n = 4 HD individuals with Vonsattel grade 4. One-way ANOVA, P = 0.029. Unpaired two-tailed t -test control versus HD2 P = 0.003; control versus HD4 P = 0.034. For C1Q in the caudate nucleus samples, n = 6 control individuals, n = 4 HD individuals with Vonsattel tissue grade 2 and n = 4 HD individuals with Vonsattel tissue grade 4. One-way ANOVA, P = 0.181. Unpaired two-tailed t -test, control versus HD2 P = 0.040, control versus HD4 P = 0.156. For C3 and C1Q in the cerebellum samples, n = 4 control individuals, n = 4 HD individuals with Vonsattel tissue grade 2 and n = 4 HD individuals with Vonsattel tissue grade 4. For C3, one-way ANOVA, P = 0.215. Unpaired two-tailed t -test, control versus HD2 P = 0.982; control versus HD4 P = 0.156. For C1q, one-way ANOVA, P = 0.981. Unpaired two-tailed t -test, control versus HD2 P = 0.978; control versus HD4 P = 0.888.
