Representative images of BODIPY-stained C. elegans were treated with 20 µg ml –1 (0.5 IU ml –1 insulin) QD-INS–CS/GS for 24 h and n = 10 independent animals were analysed. The ROI shows the anterior intestine for fat storage measurements. The compass shows the anterior, posterior, dorsal and ventral regions of worms. Scale bar, 200 µm.
