Flowchart for the gene-prioritization pipeline. The primary goal of this pipeline was to identify a single gene for each of the 121 genome-wide significant loci. Evidence of eight indicators was collected in two stages so that each prioritized gene was supported by at least one indicator from stage 1. At stage 1, we used five indicators to collect evidence for all genes within 1 Mb of index variants. This procedure identified 523 candidate genes with evidence of at least one indicator. At stage 2, evidence of three additional indicators was collected for these 523 genes. Finally, at stage 3, the above eight indicators were combined in three steps in order of precedence to identify a single prioritized gene for 121 loci.
