Fat storage was increased in the ROI (anterior intestine) in 24-h-treated QD-INS–CS/GS (0.5 IU ml –1 ) C. elegans compared with controls (OP50 fed only). Data points, mean ± s.d. shown ( n = 3 biologically independent samples ( c ), one-way ANOVA with Tukey’s correction; n = 10 biologically independent animals ( e ) and t -test with Welch correction; α = 0.05).