Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Vector Map, Polyantigen Expression, Constructs
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Vector map of the piggyBac polyantigen expression constructs used in this study. A single transcript containing 47 tandem neoantigens followed by seven control peptides and an IRES-linked mTagBFP2 (BFP) reporter is driven by an EF1a promoter. Neoantigens were either directly concatenated (no-linker) or interspersed by short flexible linker sequences (linker). b was created with BioRender.com.

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