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Open access visualization of Microscopy, Error Bars, Immunofluorescence Microscopy, mCherry/EGFP expression, Human T Cells
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Immunofluorescence microscopy showing mCherry/EGFP expression levels in human T cells at 72 h after co-transduction of dCas9 in combination with either MCP-mCherry (control), MCP-eN3x9-T2A-EGFP, MCP-MSN-T2A-EGFP, MCP-NMS-T2A-EGFP or MCP-VPR-T2A-EGFP, respectively (white scale bars, 100 m for T cells). MCP-fusion vectors also contain a U6-driven gRNA expression cassette and either a CARD9 (T cells).

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