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Synthetic zinc finger (ZF) proteins harboring indicated effector domains were designed to target the human ICAM1 promoter. Relative ICAM1 expression (bottom) at 72 h after indicated ZF fusion protein-encoding plasmids were transfected. All samples were processed for qPCR at 72 h post-transfection in HEK293T cells. See the source data for more information. Data are the result of 4 biological replicates. Data are presented as mean ± s.e.m. P values were determined using unpaired two-sided t -test. NLS, nuclear localization signal.
#Bar Plot#Zinc finger proteins#Effector domains#ICAM1 promoter#ICAM1 expression#Plasmids#qPCR#HEK293T cells#Biological replicates#Mean#SEM#T-test#NLS
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