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Multiplexed activation of 6 endogenous genes at 72 h after co-transfection of Eco-Cascade complexes when MSN was fused to Cas6 and targeted using a single crRNA array expression plasmid (1 crRNA per promoter). All samples were processed for qPCR at 72 h post-transfection in HEK293T cells. See the source data for more information. Data are the result of 4 biological replicates. Data are presented as mean ± s.e.m. P values were determined using unpaired two-sided t -test. NLS, nuclear localization signal.

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