Bar Plot from Scientific Research

B Base editing conversion rates were determined via CRISPResso2 NGS analysis following PCR amplification of MECP2 -integrated loci, in comparison to SpCas9-ABE8e and SpRYc-ABE8e for the C502T installed mutation. Samples were performed in independent nucleofection triplicates ( n = 3) SD, with the center of error bars depicting the mean. For individual samples, statistical significance was determined by a two-tailed Student’s t test, as compared to the SpCas9-ABE8e control. The exact p values for SpRY-ABE8e and SpRYc-ABE8e compared to SpCas9-ABE8e are both <0.0001. Calculated p values are represented as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant.

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