Workflow from Scientific Research

Open access visualization of Workflow, Illustration, REVeRT AAV8(Y733F) vector cassettes, Split Cas9-VPR, sgRNAs
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DOI: 10.1038/s41467-023-42386-0

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Upper panel, Dual REVeRT AAV8(Y733F) vector cassettes expressing split Cas9-VPR and three sgRNAs targeting either exon 1 of Rho (g1) or the promoter region of Opn1mw (g2–g4). A lacZ -targeting sgRNA (gL) is used as a non-targeting control. Lower panel, The corresponding dual split intein AAV8(Y733F) vector cassettes used for side-by-side comparison to REVeRT shown in ( c ).

#Workflow#Illustration#REVeRT AAV8(Y733F) vector cassettes#Split Cas9-VPR#sgRNAs#Rho#Opn1mw#lacZ-targeting sgRNA#Intein AAV8(Y733F) vector cassettes

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