Basal neuronal activity of SDGs expressing the dominant negative InR. Samples were prepared from larvae fed with normal food. Top, traces of F GCaMP /F mCherry , calculated by normalizing the signal intensity of GCaMP with that of CsChrimson::mCherry expressed as a marker. Thick lines and accompanying shades represent the median and the interquartile range, respectively. Black, +> InR DN fed; magenta, +> InR DN refed; gray, SDGs > InR DN fed; blue, SDGs > InR DN refed. Bottom, mean F GCaMP /F mCherry values from the 10-s recording windows. Box plots show the median with an interquartile range, and whiskers represent the minimum-to-maximum range. ** p < 0.005, n.s. p >= 0.05 (Mann–Whitney U-test with Bonferroni correction).
