Ca 2+ imaging during C4da optogenetic stimulation while SDGs were silenced by Kir2.1 expression. ChR2 was activated by blue-light illumination while intracellular Ca 2+ levels were measured by the red-shifted Ca 2+ indicator RGECO. Top, traces of F/F 0 . The thick lines (black, SDGs-spGAL4 > + ; magenta, SDGs-spGAL4 > UAS-Kir2.1 ) and accompanying shades represent the median and interquartile range, respectively. The blue-shaded box indicates the time window of optogenetic stimulation. Bottom, peak F/F 0 changes. In this and following panels, n indicates the number of biologically independent animals used for each group. Box plots show the median with an interquartile range, and whiskers represent the minimum-to-maximum range. ** p < 0.005 (MannWhitney U-test).
