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V 1/2 values of WT KCNQ2 and mutants. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test was applied. p and n values compared to WT ( n = 12) are p = 0.9379 and n = 7 for R87A, *** p = 0.0003 and n = 7 for R89A, p = 0.1484 and n = 9 for R214A, and ** p = 0.0015 and n = 10 for R327A. n represents the number of experiments from individual cells.
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