Transcriptomics ( a ) and serum proteomics measured by SomaO ( b ) or mesoscale platform ( c ), markers on D1 (baseline) and following initial immunization (D2) compared with boost immunization (D58) for low and high dose of Ad26.COV2.S. a heatmaps show the row-scaled expression in blood (Wald test, adjusted P < 0.05) or serum levels (limma t test, adjusted P < 0.05) of the top interferon and proinflammatory markers increased on D2 compared with D58. The barplots shown on the right side of each heatmap correspond to the log2 fold change mRNA ratio of D2/D58 for each marker. Comparison of individual marker serum levels was evaluated using a matched two-tailed Wilcoxon signed-rank test and a P value cutoff of <0.05, where *<0.05; **<0.005, and ***<0.0005. Exact P values were shown for each comparison. Red bars correspond to the median of all scores for each time point. Source data are provided as a Source data file.
