Line Plot from Scientific Research

Percentage of cells (from e) that cleared the type I-F strong (blue) or medium (orange) priming-inducing plasmids compared with a naive control (no matching protospacer, black). P. atrosepticum strains contained a second plasmid expressing RacrIF1 from the PBAD promoter (bottom) or an empty vector control (top). Flow cytometry was used to quantify the plasmid-encoded mCherry signal. Data shown represent n = 3 biological replicates plotted as mean ±s.d. Statistical significance was assessed using a two-way ANOVA test of primed samples compared with the naive control (*P ≤ 0.05).

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