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Transwell detects the migration and invasion ability of KYSE150 and KYSE410 without treatment: (i) NC + DMSO, (ii) OE + DMSO, (iii) OE + MK2206. A 10 mM stock solution was prepared by dissolving MK2206 in DMSO, where the MK22206 experimental concentration was 2 mM and 0.02% DMSO was used as a negative control. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

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