Left, CRISPR knockout (KO) screen targeting 9p21.3 genes in KP4 cells with CRISPRi knockdown using Ch2-2 gRNA (control), or PELO gRNA 1 or 2. Right, differences in Chronos gene effect scores between PELO and Ch2-2 knockdown for both PELO gRNAs. Data are mean s.e.m. of biological replicates: n = 2. Significance was calculated as follows: left-tailed Wilcoxon rank-sum test. Representative data from one experiment are shown. All experiments were performed once.
