Top, immunoblots of FOCAD, PELO and α-tubulin in MIA PaCa-2 cells. Bottom, relative viability in MIA PaCa-2 cells stably expressing indicated cDNA DOX-induced PELO knockdown. Data are mean s.e.m. of biological replicates: n = 3 except for PELO cDNA in DOX- condition, for which n = 2. Significance was calculated as follows: right-tailed Student’s t -test. Representative data from one experiment are shown. All experiments were performed twice. For immunoblots, α-tubulin was used as loading control.
