Workflow from Scientific Research

In silico workflow for data processing and analysis. RNA-seq data from the specified cohorts were downloaded from the indicated resources, processed, and normalized. Differentially expressed genes (DEGs) were identified through comparisons between tumor and normal samples. Functional enrichment analysis, including GO, KEGG, and Reactome pathway analysis, was conducted to elucidate the biological processes and pathways associated with the DEGs. Transcription factor (TF) analysis was performed to identify key TFs regulating gene expression. Finally, the identified TFs were validated through literature review and experimental data.

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