Microscopy from Scientific Research

Example of simultaneous two-color single-molecule imaging and single-particle tracking analysis at high labeling efficiency in the optimized conditions. Left: first TIRF image of the acquired movie where the same cell is simultaneously detected in the Atto 488 channel (left) and the Atto 565 channel (right). Right: superimposed yellow tracks as obtained with single-particle tracking after splitting and analyzing each channel (shown is the 50th frame with tracks reconstructed until that frame from a 100-frame movie; see also Additional file 4 : Video S3, Additional file 5 : Video S4, Additional file 6 : Video S5). Scale bars: 5 μm

DOI